A quantitative relationship between polarization properties of fluorescence light and molecular rotational diffusion was first derived by Perrin (1926). His results, which concerned spherical particles, have later been refined to the more complex rotational motion of asymmetric bodies (Memming, 1961; Chuang & Eisenthal, 1972; Ehrenberg & Rigler, 1972; Belford, Belford & Weber, 1972).
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Ehrenberg et al. (1976) studied this question.
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