The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing.
Population
Nuclear extracts and in vitro protein-RNA interaction models
Comparison
Addition of purified recombinant U2AF35 vs Nuclear extracts deficient in U2AF35
Design
Preclinical
Authors
Discussion
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Member takes
Implication
No immediate clinical implications; extends molecular models of enhancer-dependent splicing.
Structured PICO
P
Population
Nuclear extracts and in vitro protein-RNA interaction models
I
Intervention
Addition of purified recombinant U2AF35
C
Comparator
Nuclear extracts deficient in U2AF35
O
Outcome
Restoration of constitutive and enhancer-dependent splicingsurrogate
U2AF35 functions as a critical bridge between U2AF65 and the enhancer complex to recruit U2AF65 to the adjacent intron during RNA splicing.
Cite This Study
Zuo et al. (1996) studied this question.