February 1, 1991Genes & DevelopmentOpen Access

The role of branchpoint and 3'-exon sequences in the control of balanced splicing of avian retrovirus RNA.

View Full Paper

Ask AI

Bookmark

Population

Avian sarcoma-leukosis virus (ASLV) pre-mRNA (wild-type, mutant, and pseudorevertant)

Comparison

Insertion mutations and cis-acting suppressor… vs Wild-type ASLV RNA

Design

Preclinical

Authors

XFXiang‐Dong FuJohn Innes Centre RKRichard A. KatzFox Chase Cancer Center ASA M SkalkaUtah State University

Discussion

Member takes

Implication

In vitro splicing data extend mechanistic understanding of splice site mutations; leaves open relevance to mammalian systems or therapeutic targeting.

Structured PICO

Population

Avian sarcoma-leukosis virus (ASLV) pre-mRNA (wild-type, mutant, and pseudorevertant)

Intervention

Insertion mutations and cis-acting suppressor mutations (single-base changes, small deletions near 3' splice site, deletions in 3' exon)

Comparator

Wild-type ASLV RNA

Outcome

Efficiency and pattern of pre-mRNA splicing (spliceosome assembly, lariat formation, cleavage, exon ligation)

The efficiency of splicing at the env 3' splice site in ASLV can be affected at multiple levels including spliceosome assembly, lariat formation, and exon ligation.

Cite This Study

Fu et al. (1991) studied this question.

synapsesocial.com/papers/6a901e86b71d06dae589d553 https://doi.org/10.1101/gad.5.2.211

View Full Paper

Ask AI

Bookmark

February 1, 1991Genes & DevelopmentOpen Access

The role of branchpoint and 3'-exon sequences in the control of balanced splicing of avian retrovirus RNA.

View Full Paper

Ask AI

Bookmark

Population

Avian sarcoma-leukosis virus (ASLV) pre-mRNA (wild-type, mutant, and pseudorevertant)

Comparison

Insertion mutations and cis-acting suppressor… vs Wild-type ASLV RNA

Design

Preclinical

Authors

XFXiang‐Dong FuJohn Innes Centre RKRichard A. KatzFox Chase Cancer Center ASA M SkalkaUtah State University

Discussion

Member takes

Implication

In vitro splicing data extend mechanistic understanding of splice site mutations; leaves open relevance to mammalian systems or therapeutic targeting.

Structured PICO

Population

Avian sarcoma-leukosis virus (ASLV) pre-mRNA (wild-type, mutant, and pseudorevertant)

Intervention

Insertion mutations and cis-acting suppressor mutations (single-base changes, small deletions near 3' splice site, deletions in 3' exon)

Comparator

Wild-type ASLV RNA

Outcome

Efficiency and pattern of pre-mRNA splicing (spliceosome assembly, lariat formation, cleavage, exon ligation)

The efficiency of splicing at the env 3' splice site in ASLV can be affected at multiple levels including spliceosome assembly, lariat formation, and exon ligation.

Cite This Study

Fu et al. (1991) studied this question.

synapsesocial.com/papers/6a901e86b71d06dae589d553 https://doi.org/10.1101/gad.5.2.211

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark