Key result
Optimization of the suboptimal nt 3225 3' splice site counteracted the in vivo function of the exonic splicing suppressor and led to preferential selection of the nt 3225 3' splice site.
Population
in vitro HeLa nuclear extracts and in vivo human 293 cells transfected with BPV-1 late minigene plasmids
Comparison
Optimization of the suboptimal nt 3225 3' splice… vs Wild-type BPV-1 late pre-mRNA constructs with…
Design
Preclinical
Authors
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Establishes dependence of BPV-1 splicing suppressor on suboptimal splice site; leaves open extension to human viruses or therapeutic targeting.
The function of the BPV-1 exonic splicing suppressor requires a suboptimal upstream 3' splice site, and optimizing this site counteracts the suppressor's effect.
Zheng et al. (2000) studied Bovine Papillomavirus Type 1 (BPV-1) alternative splicing. Optimization of a weak 3' splice site vs. Wild-type BPV-1 late pre-mRNA was evaluated on Alternative splicing pattern (utilization of nt 3225 vs nt 3605 3' splice site). Optimization of the suboptimal nt 3225 3' splice site counteracted the in vivo function of the exonic splicing suppressor and led to preferential selection of the nt 3225 3' splice site.
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