What question did this study set out to answer?

This research aims to investigate the structure and expression dynamics of the firefly luciferase gene in mammalian cells.

February 1, 1987Molecular and Cellular BiologyOpen Access

Firefly luciferase gene: structure and expression in mammalian cells.

View Full Paper

Ask AI

Bookmark

Authors

JWJeffrey R. de WetUniversity of Michigan KWKeith V. WoodnLIGHT (United States)MDM. DeLucaRobert Bosch (Germany)

Discussion

Member takes

Implication

Randomized trial investigates luciferase expression influenced by genetic modifications in cells, suggesting enhanced monitoring of promoter activity.

Key Points

This research aims to investigate the structure and expression dynamics of the firefly luciferase gene in mammalian cells.
Determined the nucleotide sequence of luciferase from Photinus pyralis using cDNA and genomic clones.
Inserted the full-length intronless luciferase gene into mammalian expression vectors and introduced it into monkey (CV-1) cells.
Assessed the impact of deleting the 5'-untranslated region on luciferase expression levels.
Transgenic monkey cells showed transient expression of enzymatically active firefly luciferase.
Deletion of the 5'-untranslated region resulted in a twofold increase in luciferase expression.
The luciferase assay demonstrated 30- to 1,000-fold greater sensitivity compared to chloramphenicol acetyltransferase expression.

Cite This Study

Wet et al. (1987) studied this question.

synapsesocial.com/papers/6a06801af2cf2ebbc4025891 https://doi.org/10.1128/mcb.7.2.725

View Full Paper

Ask AI

Bookmark

Implication

Randomized trial investigates luciferase expression influenced by genetic modifications in cells, suggesting enhanced monitoring of promoter activity.