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January 1, 1984Nucleic Acids ResearchOpen Access

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

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Authors

DMDouglas A. MeltonBroad InstitutePKPaul A. KriegThe Royal Melbourne HospitalMRMichael R. RebagliatiUniversity of Iowa

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Implication

Randomized trial demonstrates effective RNA probe synthesis using an SP6 promoter, implying enhanced sensitivity in hybridization methods.

Key Points

  • The aim is to develop an efficient method for producing biologically active RNA and RNA probes from plasmids with an SP6 promoter.
  • Constructed cloning vectors containing an SP6 promoter upstream of a polylinker sequence.
  • Established optimal conditions for in vitro RNA synthesis using SP6 RNA polymerase.
  • Demonstrated RNA probes' effectiveness in nucleic acid hybridization techniques.
  • Single stranded RNA probes demonstrated high specific activity.
  • RNA probes prepared significantly enhanced sensitivity in hybridization methods.
  • The transcription system also allowed preparation of RNA substrates for RNA processing and translation studies.

Cite This Study

Melton et al. (1984) studied this question.

synapsesocial.com/papers/6a07b9a0e83cdb3dbe79fdf6https://doi.org/10.1093/nar/12.18.7035
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