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This research aims to characterize how palmitate impairs fatty acid oxidation and citric acid cycle activity in neonatal cardiomyocytes.

Lipotoxic Palmitate Impairs the Rate of β-Oxidation and Citric Acid Cycle Flux in Rat Neonatal Cardiomyocytes

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This research aims to characterize how palmitate impairs fatty acid oxidation and citric acid cycle activity in neonatal cardiomyocytes.
Measured fatty acid oxidation and acetyl-CoA oxidation in primary rat neonatal cardiomyocytes.
Assessed enzyme activities including aconitase and isocitrate dehydrogenase.
Evaluated reactive oxygen species and diacylglycerol levels using specific assays.
Palmitate significantly impaired mitochondrial β-oxidation and citric acid cycle flux.
Decreased net aconitase and isocitrate dehydrogenase activities were observed; oxidative stress was not the cause.
Impairment may result from diacylglycerol-mediated PKC activation.

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Should not guide diabetes management; leaves open translation of neonatal cardiomyocyte findings to adult human hearts.

Haffar et al. (2016) studied this question.

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