What question did this study set out to answer?

To determine whether the two actin monomers in the ternary gelsolin-actin complex adopt the parallel orientation characteristic of the F-actin genetic helix.

December 1, 1993European Journal of BiochemistryOpen Access

The actin monomers in the ternary gelsolin: 2 actin complex are in an antiparallel orientation

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Authors

THThomas HesterkampGerman Center for Infection Research AWAlan G. WeedsDartmouth College HMHans Georg MannherzSt. Josef-Hospital

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Implication

Biochemical cross-linking analysis demonstrates antiparallel alignment of actin monomers in ternary gelsolin complexes, indicating structural flexibility rather than a fixed filament-like...

Key Points

To determine whether the two actin monomers in the ternary gelsolin-actin complex adopt the parallel orientation characteristic of the F-actin genetic helix.
Performed chemical cross-linking of gelsolin-actin complexes, actin paracrystals, and F-actin using N,N'-1,4-phenylenedimaleimide.
Characterized cross-linked species by molecular mass (86 kDa lower dimer vs. 115 kDa upper dimer) and mapped contact sites.
Assessed actin polymerization nucleation activity and binding stoichiometry using isolated dimers and gelsolin truncation fragments.
Cross-linking of the ternary gelsolin:2 actin complex exclusively yielded an 86 kDa lower dimer mediated by a Cys374-Cys374 bond, reflecting an antiparallel monomer orientation.
Addition of intact gelsolin to F-actin specifically inhibited the formation of the 115 kDa upper dimer associated with helical actin filaments.
Isolated lower dimer bound gelsolin at a 1:1 stoichiometry and inhibited nucleation, whereas gelsolin segment 2-6 uniquely induced the 115 kDa upper dimer under nucleating conditions.

Cite This Study

Hesterkamp et al. (1993) studied this question.

synapsesocial.com/papers/6a088e3f7f7fcd1344ddec81 https://doi.org/10.1111/j.1432-1033.1993.tb18403.x

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Implication

Biochemical cross-linking analysis demonstrates antiparallel alignment of actin monomers in ternary gelsolin complexes, indicating structural flexibility rather than a fixed filament-like...

The actin monomers in the ternary gelsolin: 2 actin complex are in an antiparallel orientation

Discussion

Member takes

Implication

Key Points

Cite This Study

The actin monomers in the ternary gelsolin: 2 actin complex are in an antiparallel orientation

Discussion

Member takes

Implication

Key Points

Cite This Study

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