June 13, 1989Biochemistry

End-label fingerprintings show that the N- and C-termini of actin are in the contact site with gelsolin

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Population

Actin and gelsolin proteins/fragments

Design

Preclinical

Authors

KSKazuo SutohWaseda University HYHelen L. YinThe University of Texas Southwestern Medical Center

Discussion

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Implication

Preclinical binding data warrant no clinical changes; extends actin-gelsolin structural models but leaves functional roles open.

Structured PICO

Population

Actin and gelsolin proteins/fragments

Intervention

Cleavage of gelsolin by chymotrypsin or thermolysin, complexing with actin, and cross-linking with 1-ethyl-3-[3-(dimethylamino)prophyl]carbodiimide (EDC)

Outcome

Location of cross-linked bonds along the actin sequence

The study maps the contact sites between gelsolin and actin, showing that the N- and C-termini of actin are involved in binding gelsolin.

Cite This Study

Sutoh et al. (1989) studied this question.

synapsesocial.com/papers/6a8d18a6952274da7f0e562e https://doi.org/10.1021/bi00438a052

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Bookmark

End-label fingerprintings show that the N- and C-termini of actin are in the contact site with gelsolin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

End-label fingerprintings show that the N- and C-termini of actin are in the contact site with gelsolin

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider