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September 1, 1990Blood

Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured human endothelial cells published erratum appears in Blood 1990 Dec 1;76(11):2420

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Authors

TCTM CarlosUniversity of WashingtonBSBR SchwartzUniversity of WashingtonNKNL KovachUniversity of Washington

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Implication

In vitro study demonstrates VCAM-1 mediates CD18-independent lymphocyte adhesion to cytokine-stimulated endothelium, suggesting a distinct pathway for immune cell recruitment during inflammation.

Key Points

  • To characterize the endothelial expression and adhesive function of vascular cell adhesion molecule-1 (VCAM-1) following cytokine stimulation.
  • Measured VCAM-1 surface expression on cultured human umbilical vein endothelium (HUVE), dermal fibroblasts, arterial smooth muscle cells, and keratinocytes stimulated with rhTNF-alpha, rhIL-1, lipopolysaccharide, or rhIFN-gamma using ELISA and radioimmunoprecipitation.
  • Evaluated the adherence of peripheral blood lymphocytes, lymphocytic cell lines, and neutrophils to rhTNF-activated HUVE in the presence of functional blocking monoclonal antibody MoAb 4B9 alone and in combination with CD18 MoAb 60.3.
  • VCAM-1 was minimally expressed on unstimulated HUVE but was rapidly induced by rhTNF-alpha, rhIL-1, and lipopolysaccharide; it was not induced on dermal fibroblasts, arterial smooth muscle cells, or keratinocytes.
  • MoAb 4B9 significantly inhibited the adherence of peripheral blood lymphocytes and lymphocytic cell lines to rhTNF-activated HUVE without inhibiting neutrophil adherence.
  • Simultaneous treatment with MoAb 4B9 and anti-CD18 MoAb 60.3 resulted in additive inhibition of lymphocyte adherence to activated HUVE, confirming a CD18-independent pathway.

Cite This Study

Carlos et al. (1990) studied this question.

synapsesocial.com/papers/6a08908ead370a6b44de2fc1https://doi.org/10.1182/blood.v76.5.965.965
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Also Consider

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  1. 1Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon gamma and is mediated by intercellular adhesion molecule 1 (ICAM-1).1988 · 677 citations
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  3. 3Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells.1988 · 991 citations
  4. 4Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1).1986 · 2,472 citations
  5. 5Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors.1981 · 299 citations