What question did this study set out to answer?

To determine whether exosome-like vesicles mediate Wingless secretion and morphogen gradient formation in Drosophila wing discs.

October 5, 2012TrafficOpen Access

Drosophila S2 Cells Secrete Wingless on Exosome‐Like Vesicles but the Wingless Gradient Forms Independently of Exosomes

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Authors

KBKaren BeckettDublin City University SMSolange MonierCentre National de la Recherche Scientifique LPLucy PalmerUniversity of Central Asia

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Implication

Experimental study demonstrates that Wingless spreads independently of exosomes in Drosophila wing discs, indicating alternative mechanisms drive morphogen gradient formation.

Key Points

To determine whether exosome-like vesicles mediate Wingless secretion and morphogen gradient formation in Drosophila wing discs.
Analyzed conditioned medium from Drosophila S2 cells expressing Wingless using proteomic profiling and signaling activation assays.
Conducted cell-based RNAi screens to identify factors regulating exosome release, identifying the small GTPase Rab11.
Examined Wingless distribution and extracellularly tagged Evi localization in vivo following Rab11 knockdown in wing imaginal discs.
Wingless co-fractionated with signaling-competent, exosome-like vesicles enriched in Evi and conserved exosomal marker proteins in S2 cell culture.
Rab11 knockdown significantly reduced exosome production in cell assays but failed to perturb Wingless secretion or gradient formation in vivo.
Extracellularly tagged Evi expressed from a bacterial artificial chromosome was retained and not released from Wingless-producing imaginal disc cells.

Cite This Study

Beckett et al. (2012) studied this question.

synapsesocial.com/papers/6a0891b6a8a781e4cde8a1d1 https://doi.org/10.1111/tra.12016

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Implication

Experimental study demonstrates that Wingless spreads independently of exosomes in Drosophila wing discs, indicating alternative mechanisms drive morphogen gradient formation.

Drosophila S2 Cells Secrete Wingless on Exosome‐Like Vesicles but the Wingless Gradient Forms Independently of Exosomes

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Implication

Key Points

Cite This Study

Drosophila S2 Cells Secrete Wingless on Exosome‐Like Vesicles but the Wingless Gradient Forms Independently of Exosomes

Discussion

Member takes

Implication

Key Points

Cite This Study

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