Key result
Single-cell analysis reveals Clec11a+ perivascular adipose stem cells potentially regulate smooth muscle cell differentiation.
Why the study?
The heterogeneity of subcutaneous and abdominal ADSCs has been well addressed, but perivascular ADSC heterogeneity has not been investigated.
This study identifies distinct subpopulations of perivascular adipose-derived stem cells, highlighting the role of the Clec11a+ subpopulation in smooth muscle cell differentiation and vascular remodeling.
Hypothesis-generating for perivascular stem cell roles in diabetic vascular remodeling; human validation required before any clinical consideration.
Adipose-derived stem cells (ADSCs) can differentiate into vascular lineages and participate in vascular remodeling. Perivascular ADSCs (PV-ADSCs) draw attention because of their unique location. The heterogeneity of subcutaneous (SUB) and abdominal ADSCs were well addressed, but PV-ADSCs' heterogeneity has not been investigated. In this study, we applied single-cell analysis to compare SUB-ADSCs and PV-ADSCs regarding their subpopulations, functions, and cell fates. We uncovered four subpopulations of PV-ADSCs (Dpp4+, Col4a2+/Icam1+, Clec11a+/Cpe+, and Sult1e1+ cells), among which the Clec11a+ subpopulation potentially participated in and regulated PV-ADSC differentiation toward a smooth muscle cell (SMC) phenotype. Distinct characteristics between PV-ADSCs and SUB-ADSCs were revealed.
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Xie et al. (2021) studied this question. Single-cell analysis of perivascular adipose-derived stem cells (PV-ADSCs) vs. Subcutaneous adipose-derived stem cells (SUB-ADSCs) was evaluated on Subpopulations, functions, and cell fates. Single-cell analysis revealed four subpopulations of perivascular adipose-derived stem cells, with the Clec11a+ subpopulation potentially regulating differentiation toward a smooth muscle cell phenotype.
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