Key result
Losartan and angiotensin 1-7 cut ischemia-reperfusion kidney damage ~73% in ovariectomized rats without estradiol.
Why the study?
Most tissue damages after ischemia-reperfusion (IR) are due to activation of the renin-angiotensin system (RAS), prompting evaluation of the interaction of sex hormones and RAS in ovariectomized rats subjected to IR.
Does estradiol combined with losartan or Ang 1-7 improve kidney damage in ovariectomized rats subjected to ischemia-reperfusion?
Does estradiol combined with losartan or Ang 1-7 improve kidney damage in ovariectomized rats subjected to ischemia-reperfusion?
Absolute Event Rate: 1.1% vs 4%
p-value: p=<0.05
In a rat model of renal ischemia-reperfusion injury, estrogen exacerbated renal damage, whereas blocking the AT1 receptor or administering Ang 1-7 in the absence of estrogen improved kidney function.
BACKGROUNDS: Ischemia-reperfusion (IR) injuries occur in a variety of clinical conditions, which lead to kidney damage. Most of the tissue damages after IR are due to the activation of the renin-angiotensin system (RAS). Hence, in this study, the interaction of sex hormones and RAS in ovariectomized (OV) rats subjected to IR induction has been studied. MATERIALS AND METHODS: The animals were divided into different groups. Groups 1 (OV + E, OV rat + estradiol) and 2 (OV rat) each one consisted of three separate IR-induced subgroups treated with losartan, angiotensin 1-7 (Ang 1-7), and their combination, Group 3, as control and Group 4, as sham. Next, 72 h after IR, blood samples were collected, the right kidneys were homogenized, and left kidneys were fixed in 10% formalin. RESULTS: < 0.05). Mean serum levels of these factors in OV + E groups are higher than those of the OV. The presence or absence of estradiol did not affect the levels of antioxidants in the different groups receiving Los, Ang 1-7, and their combination. Los, Ang 1-7, and their combination reduced serum and kidney malondialdehyde levels in both OV and OV + E groups. CONCLUSION: Estrogen not only fails to improve renal functioning but it can also exacerbate it. While the treatments used in this study, in the absence of estradiol, it had a better effect on kidney damages and improved its functions.
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Safari et al. (2019) studied Ischemia-reperfusion injury (n=64). Losartan, Angiotensin 1-7, and their combination (with or without estradiol) vs. Control (saline) and Sham was evaluated on Kidney tissue damage score (KTDS) (p=<0.05). In ovariectomized rats subjected to ischemia-reperfusion, estradiol exacerbated renal injury, whereas losartan, angiotensin 1-7, and their combination significantly reduced kidney tissue damage in the absence of estradiol.
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