What question did this study set out to answer?

To evaluate the performance of droplet digital PCR (ddPCR) for quantifying rare HIV DNA targets, including total viral DNA and episomal 2-LTR circles, in clinical specimens.

April 3, 2013PLoS ONEOpen Access

Highly Precise Measurement of HIV DNA by Droplet Digital PCR

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Authors

MSMatthew C. Strain SLSteven M. Lada TLTiffany Luong

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Overview

Comparative validation reveals superior precision of droplet digital PCR for quantifying rare HIV DNA targets in patient samples, highlighting its utility for evaluating latent viral reservoirs.

Key Points

To evaluate the performance of droplet digital PCR (ddPCR) for quantifying rare HIV DNA targets, including total viral DNA and episomal 2-LTR circles, in clinical specimens.
Analyzed over 300 clinical samples from HIV-infected patients receiving combination antiretroviral therapy (cART).
Directly compared ddPCR against real-time PCR (qPCR) across more than 150 clinical samples assayed in triplicate to quantify pol copies per million cells and 2-LTR circles.
ddPCR achieved an average 5-fold reduction in the coefficient of variation for HIV pol copy numbers compared with qPCR.
Quantification of episomal 2-LTR circles demonstrated a >20-fold accuracy improvement using ddPCR relative to qPCR.
ddPCR enabled absolute target quantification without standard curves and showed relative tolerance to sequence mismatches in primers and probes.

Cite This Study

Strain et al. (2013) studied this question.

synapsesocial.com/papers/6a0959deb0d552aa8b45a246 https://doi.org/10.1371/journal.pone.0055943

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April 3, 2013PLoS ONEOpen Access

Highly Precise Measurement of HIV DNA by Droplet Digital PCR

View Full Paper

Ask AI

Bookmark

Authors

MSMatthew C. Strain SLSteven M. Lada TLTiffany Luong

Discussion

Member takes

Overview

Comparative validation reveals superior precision of droplet digital PCR for quantifying rare HIV DNA targets in patient samples, highlighting its utility for evaluating latent viral reservoirs.

Key Points

To evaluate the performance of droplet digital PCR (ddPCR) for quantifying rare HIV DNA targets, including total viral DNA and episomal 2-LTR circles, in clinical specimens.
Analyzed over 300 clinical samples from HIV-infected patients receiving combination antiretroviral therapy (cART).
Directly compared ddPCR against real-time PCR (qPCR) across more than 150 clinical samples assayed in triplicate to quantify pol copies per million cells and 2-LTR circles.
ddPCR achieved an average 5-fold reduction in the coefficient of variation for HIV pol copy numbers compared with qPCR.
Quantification of episomal 2-LTR circles demonstrated a >20-fold accuracy improvement using ddPCR relative to qPCR.
ddPCR enabled absolute target quantification without standard curves and showed relative tolerance to sequence mismatches in primers and probes.

Cite This Study

Strain et al. (2013) studied this question.

synapsesocial.com/papers/6a0959deb0d552aa8b45a246 https://doi.org/10.1371/journal.pone.0055943

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