Early detection and rapid diagnosis of circulating HPV-DNA detected using droplet digital PCR

• Compared to standard PCR, ddPCR has higher sensitivity and specificity for detecting low-abundance HPV-DNA. • It correctly measures viral loads, distinguishes between transitory and persistent infections, provides prognostic information, and allows for real-time surveillance of HPV-related malignancies. • Its high accuracy reduces false results, which improves diagnostic reliability and patient management.