Randomized trial investigates extracellular vesicle effects on blastocyst development in bovine embryos, suggesting improved methods for culture strategies.
Key Points
The study aims to investigate the effects of oviductal and uterine extracellular vesicles on bovine embryo development in vitro.
Presumptive zygotes were cultured in groups or individually under varying embryo densities.
Embryos were exposed to oviductal EVs from days 1-4, followed by uterine EVs from days 5-8.
Evaluation focused on blastocyst rates, cell counts, and lipid droplet accumulation.
Sequential EV exposure increased blastocyst rates and mean cell numbers in individually cultured embryos.
Accumulation of large lipid droplets was reduced in individually cultured embryos due to EV exposure.
Sequential EVs exposure reduced apoptotic cells and lipid areas in both individually and group cultured embryos.