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August 2, 2021African Health SciencesOpen Access

Streptozotocin-induced type 1 and 2 diabetes in rodents: a model for studying diabetic cardiac autonomic neuropathy

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Key result

STZ-based regimens induce diabetes and cardiac autonomic neuropathy in up to ~89% of rats.

  • n=84

Why the study?

Conflicting regimens exist for diabetes induction in animal models, motivating a direct comparison of high-dose streptozotocin versus multiple low doses after a high-fat diet on diabetic cardiac autonomic neuropathy.

Design

Animal experimental study

Authors

OAOlawale Mathias AkinladeNew Cross HospitalBOBamidele Victor OwoyeleUniversity of IlorinASAyodele O. SoladoyeBowen University

Discussion

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Overview

STZ-based rat models effectively induce diabetic cardiac autonomic neuropathy; extends preclinical tools but leaves human translation open.

Structured PICO

P
Population
84 Wistar rats
I
Intervention
T1DM induction (single dose streptozotocin 50mg/kg) or T2DM induction (high fat diet for 8 weeks with streptozotocin 25mg/Kg daily for five days)
C
Comparator
Control group
O
Outcome
Diabetic cardiac autonomic neuropathy (DCAN) features assessed using invasive biomarkers, histology patterns, and cardiac nerve densitiessurrogate

High single dose of streptozotocin and high fat diet with multiple low doses of streptozotocin are effective models for studying diabetic cardiac autonomic neuropathy in T1DM and T2DM rat models, respectively.

Limitations

  • Animal models may not perfectly replicate human disease progression
  • Differences in high-fat diet composition compared to other studies may affect lipid profiles

Cite This Study

Akinlade et al. (2021) studied Diabetic cardiac autonomic neuropathy (DCAN) (n=84). Streptozotocin (STZ) and High-Fat Diet (HFD) vs. Sham control / Standard chow diet was evaluated on Diabetes induction rate (blood glucose >16mmol/L) in T2DM model. High single dose of STZ and a high-fat diet combined with multiple low doses of STZ successfully induced type 1 and type 2 diabetes with cardiac autonomic neuropathy in 76% and 89% of rats, respectively.

synapsesocial.com/papers/6a09878b16dfdfe7ed342ab2https://doi.org/10.4314/ahs.v21i2.30
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Update on the Impact, Diagnosis and Management of Cardiovascular Autonomic Neuropathy in Diabetes: What Is Defined, What Is New, and What Is Unmet2019 · 305 citations
  2. 2Tests for Early Diagnosis of Cardiovascular Autonomic Neuropathy: Critical Analysis and Relevance2013 · 51 citations
  3. 3Changes in pancreatic histology, insulin secretion and oxidative status in diabetic rats following treatment with Ficus deltoidea and vitexin2017 · 118 citations
  4. 4The Characterization of High‐Fat Diet and Multiple Low‐Dose Streptozotocin Induced Type 2 Diabetes Rat Model2008 · 673 citations
  5. 5Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review)2014 · 261 citations