Key result
Microinjection of the active 61-kD catalytic fragment of MLCK into mitotic cells delayed transit time to anaphase onset and increased motile surface activity, but did not prevent cytokinesis.
Why the study?
Does microinjection of the catalytic fragment of MLCK affect mitosis and cytokinesis in dividing cells?
Population
Mitotic cells (dividing cells)
Comparison
Microinjection of the 61-kD catalytic fragment… vs Control experiments with intact MLCK or inactive…
Design
Preclinical
Authors
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Should not inform clinical practice; leaves open additional controls of cytokinesis in mammalian systems.
Does microinjection of the catalytic fragment of MLCK affect mitosis and cytokinesis in dividing cells?
Unregulated MLCK activity affects mitosis and cortical activities but does not alter the rate of cytokinesis, suggesting additional control mechanisms are involved.
Fishkind et al. (1991) studied Mitosis and cytokinesis in dividing cells. Microinjection of the 61-kD catalytic fragment of myosin light chain kinase (MLCK) vs. Intact MLCK or inactive catalytic fragment was evaluated on Effects on mitosis and cytokinesis (transit time to anaphase onset, motile surface activity, cleavage furrow formation). Microinjection of the active 61-kD catalytic fragment of MLCK into mitotic cells delayed transit time to anaphase onset and increased motile surface activity, but did not prevent cytokinesis.
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