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October 1, 1982The Journal of PhysiologyOpen Access

Studies of calcium channels in rat clonal pituitary cells with patch electrode voltage clamp

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Key result

Patch electrode voltage-clamp analysis of rat clonal pituitary cells revealed single Ca channel current amplitudes of ~0.2 pA at 25 mM-Ba2+ and ~0.7 pA at 100 mM-Ba2+.

Population

Tissue cultured clonal cells (GH(3)) isolated from a rat anterior pituitary tumour

Design

Preclinical

Authors

SHSusumu HagiwaraNihon UniversityHOHarunori OhmoriKyoto University

Discussion

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Implication

No immediate clinical implications; leaves open translation of these Ca channel properties to human physiology.

Structured PICO

P
Population
Tissue cultured clonal cells (GH(3)) isolated from a rat anterior pituitary tumour
I
Intervention
Patch electrode voltage-clamp technique with varying external cations (Ba2+, Sr2+, Ca2+) and concentrations
O
Outcome
Properties of the Ca channel including selectivity ratios, activation kinetics, and single channel current amplitudesurrogate

This study characterizes the electrophysiological properties, selectivity, and single-channel conductance of calcium channels in rat clonal pituitary cells.

Cite This Study

Hagiwara et al. (1982) studied Rat anterior pituitary tumour. Patch electrode voltage-clamp technique was evaluated on Properties of the Ca channel. Patch electrode voltage-clamp analysis of rat clonal pituitary cells revealed single Ca channel current amplitudes of ~0.2 pA at 25 mM-Ba2+ and ~0.7 pA at 100 mM-Ba2+.

synapsesocial.com/papers/6a0a1d514b13cba792519567https://doi.org/10.1113/jphysiol.1982.sp014371
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Electrical Excitability in the Rat Clonal Pituitary Cell and Its Relation to Hormone Secretion1979 · 51 citations
  2. 2Voltage-dependent inactivation of a calcium channel.1981 · 78 citations
  3. 3Internal effects of divalent cations on potassium permeability in molluscan neurones.1979 · 194 citations
  4. 4Membrane Currents Carried by Ca, Sr, and Ba in Barnacle Muscle Fiber During Voltage Clamp1974 · 239 citations
  5. 5Separation of sodium and calcium currents in the somatic membrane of mollusc neurones. With an Appendix by Yu A. Shakhovalov1977 · 363 citations