Bioavailable human metabolites of a keratin-derived hydrolysate promote primary human dermal fibroblast activities and protect against oxidative stress-related damages
Randomized trial demonstrates fibroblast activity enhancement in men after ingesting keratin hydrolysate, suggesting benefits for skin health.
Key Points
This study examines the impact of bioavailable metabolites from keratin hydrolysate on human dermal fibroblast functions and oxidative stress protection.
Ten healthy men ingested 5,000 mg of keratin hydrolysate (Kera-Diet®).
Plasma amino acids were measured using UPLC–MS over 240 minutes to identify absorption peaks.
Fibroblasts were exposed to naïve or enriched serum to assess cellular viability, migration, and oxidative stress responses.
Circulating amino acids peaked approximately 20 minutes after ingestion.
Enriched serum increased glycosaminoglycan release by 11% and hyaluronan by 43%, enhancing fibroblast migration.
Under oxidative stress, Kera-Diet® significantly reduced ROS and apoptosis while maintaining fibroblast viability.