Association of angiotensin II and receptors in peri-implantation endometrium with microvessel density and pregnancy outcomes of women with recurrent implantation failure after embryo transfer

Figures

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  Figure 1. Ang II, AT1R, and AT2R expression in endometrium between fertile controls, RM and RIF. (A) Representative images of immunohistochemical staining of Ang II, AT1R, and AT2R among groups. LE, luminal epithelium; GE, glandular epithelium; ST, stroma cells. Scale bar=100 μm. (B) Corresponding quantitative analysis. Data are presented as mean ± SEM for AngII, AT1R, and AT2R and median (range) for stromal AT1R/AT2R expression ratios in all compartments of endometrium. *p < 0.05, ***p<0.001, NS, not significant.

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  Figure 2. Arteriole and capillary density in endometrium between fertile controls, RM, and RIF. (A) Representative images of arterioles stained by AT2R and capillary stained by vWF (arrows) among groups. Scale bar = 100 μm. (B) Corresponding quantitative analysis. Data are presented as mean ± SEM. *p<0.05; NS, not significant. (C) Correlation analysis of stromal AT1R/AT2R expression ratio with arteriole density in control (r=-0.559, p=0.002), RM (r=-0.437, p=0.001) and RIF (r=-0.419, p=0.007), and its correlation with capillary density in control (r=0.256, p=0.197), RM (r=-0.227, p=0.158), and RIF (r=-0.157, p=0.334).

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  Figure 3. AT1R and AT2R expression in endometrium between women who did (Pregnant) and did not conceive (Non-pregnant) after euploid embryos transfer. (A) Representative images of immunohistochemical staining of AT1R and AT2R are shown on the left. LE=luminal epithelium; GE=glandular epithelium; ST=stroma cells. Scale bar=100 μm. Corresponding quantitative results between groups are shown on the right. Data are presented as mean ± SEM. (B) Representative images of arterioles stained by AT2R (arrows) are shown on the left. Scale bar=50 μm. Corresponding quantitative analysis between groups is shown on the right. Data are presented as mean ± SEM. (C) Comparison of the expression AT1R/AT2R ratio in all compartments of endometrium between the pregnant and non-pregnant groups is shown on the left. Data are presented as median (range). Correlation between stromal AT1R/AT2R ratio with arteriole density in the pregnant group (r=-0.438, p=0.036) and the non-pregnant group (r=-0.577, p=0.001) is shown on the right. *p<0.05, **p<0.01, ***p<0.001, NS, not significant.