Key result
Mutation of a conserved active site lysine to arginine in a model RNA virus polymerase produced an attenuated, genetically stable virus that elicited a protective immune response.
Population
Model RNA virus and its polymerase
Design
Preclinical
Authors
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May guide rational attenuation strategies for RNA virus vaccines; leaves open translation from model systems to human pathogens.
Mutation of a conserved active site lysine residue in viral polymerases may provide a universal, mechanism-based strategy for viral attenuation and vaccine development.
Weeks et al. (2012) studied Viral infection. Mutation of active site lysine to arginine in viral polymerase was evaluated on Viral attenuation, genetic stability, and protective immune response. Mutation of a conserved active site lysine to arginine in a model RNA virus polymerase produced an attenuated, genetically stable virus that elicited a protective immune response.
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