What question did this study set out to answer?

To develop an in vivo labeling system that enables the visualization and tracking of myelomonocytic cell differentiation from multipotent hematopoietic progenitors using time-lapse fluorescence microscopy.

July 15, 2000Blood

Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages

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Authors

NFNicole FaustAlbert Einstein College of Medicine FVFlorencio VarasCentre for Genomic Regulation LKLouise KellySt. James's Hospital

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Implication

In vivo genetic study demonstrates lineage-specific fluorescent labeling of myelomonocytic cells in mice, highlighting a novel system for tracking hematopoietic differentiation.

Key Points

To develop an in vivo labeling system that enables the visualization and tracking of myelomonocytic cell differentiation from multipotent hematopoietic progenitors using time-lapse fluorescence microscopy.
Targeted the enhanced green fluorescent protein (EGFP) gene into the murine lysozyme M locus via homologous recombination using a vector containing a loxP-flanked neomycin resistance cassette.
Bred targeted mice with a Cre-deleter strain to excise the neomycin cassette and analyzed blood and bone marrow lineages for fluorescence.
Lys-EGFP mice showed selective green fluorescence in myelomonocytic cells, predominantly in mature neutrophil granulocytes, while other hematopoietic lineages remained non-fluorescent.
Cre-mediated excision of the neomycin selection cassette significantly elevated the fluorescence intensity of the labeled cells.
Homozygous disruption of the lysozyme M gene did not impair development, and the resulting mice were fertile.

Cite This Study

Faust et al. (2000) studied this question.

synapsesocial.com/papers/6a0cc786c6fb28010734c4cc https://doi.org/10.1182/blood.v96.2.719

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Implication

In vivo genetic study demonstrates lineage-specific fluorescent labeling of myelomonocytic cells in mice, highlighting a novel system for tracking hematopoietic differentiation.

Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages

Discussion

Member takes

Implication

Key Points

Cite This Study

Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages

Discussion

Member takes

Implication

Key Points

Cite This Study

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