Key result
An in vitro assay using myosin-coated beads demonstrated that skeletal muscle myosin moves along actin filaments at 2-6 micron/s, dependent on ATP, pH, and ion concentrations.
This in vitro assay provides a rapid, reproducible method for quantitating the ATP-dependent movement of myosin molecules on actin.
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Enables quantitative in vitro analysis of myosin motility; leaves open translation to cardiac muscle physiology.
Sheetz et al. (1984) studied In vitro myosin movement. In vitro quantitative assay was evaluated on Myosin-bead velocity. An in vitro assay using myosin-coated beads demonstrated that skeletal muscle myosin moves along actin filaments at 2-6 micron/s, dependent on ATP, pH, and ion concentrations.
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