What question did this study set out to answer?

To identify and characterize cytosolic serine/threonine protein kinases activated in quiescent Swiss 3T3 cells following stimulation with epidermal growth factor.

July 1, 1990Journal of Biological ChemistryOpen Access

Identification of multiple epidermal growth factor-stimulated protein serine/threonine kinases from Swiss 3T3 cells.

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Authors

NANatalie G. AhnBrigham Young University JWJames E. WeielDurham University CCC. P. ChanUniversiti Putra Malaysia

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Implication

In vitro study identifies multiple epidermal growth factor-stimulated serine/threonine kinases in Swiss 3T3 cells, indicating a complex signaling cascade regulated by phosphorylation.

Key Points

To identify and characterize cytosolic serine/threonine protein kinases activated in quiescent Swiss 3T3 cells following stimulation with epidermal growth factor.
Treated quiescent Swiss 3T3 cells with epidermal growth factor for 5 minutes and harvested cytosolic extracts under conditions inhibitory to calcium- and cyclic nucleotide-dependent kinases.
Fractionated extracts using Mono Q fast protein liquid chromatography followed by sizing gel chromatography.
Assayed kinase activities against substrates including S6 peptide, 40S ribosomal S6 protein, glycogen synthase, microtubule-associated protein 2, and myelin basic protein, alongside phosphatase sensitivity tests.
Resolved eight initial kinase activity peaks on Mono Q chromatography, of which five were stimulated by epidermal growth factor treatment.
Identified at least seven distinct stimulated kinase fractions across sizing chromatography, including five S6-phosphorylating activities and two targeting microtubule-associated protein 2 and myelin basic protein.
Demonstrated that several stimulated kinase activities were inactivated by protein phosphatases, indicating regulation through upstream phosphorylation.

Cite This Study

Ahn et al. (1990) studied this question.

synapsesocial.com/papers/6a0cd0a03fce92745334d0fb https://doi.org/10.1016/s0021-9258(19)38423-6

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Implication

In vitro study identifies multiple epidermal growth factor-stimulated serine/threonine kinases in Swiss 3T3 cells, indicating a complex signaling cascade regulated by phosphorylation.

Identification of multiple epidermal growth factor-stimulated protein serine/threonine kinases from Swiss 3T3 cells.

Discussion

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Implication

Key Points

Cite This Study

Identification of multiple epidermal growth factor-stimulated protein serine/threonine kinases from Swiss 3T3 cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

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