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August 1, 1985Journal of Biological ChemistryOpen Access

Epidermal growth factor-mediated activation of an S6 kinase in Swiss mouse 3T3 cells.

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Authors

INIlse Novak‐HoferUniversity of GenevaGTGeorge ThomasBroad Institute

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Implication

Randomized trial demonstrates EGF's rapid activation of S6 kinase in Swiss mouse 3T3 cells, suggesting impact on protein synthesis.

Key Points

  • This research aims to investigate the effect of epidermal growth factor (EGF) on S6 kinase activation in Swiss mouse 3T3 cells.
  • Analyzed the potency of EGF-stimulated extracts for phosphorylating S6 compared to quiescent cells.
  • Examined the effect of various phosphatase inhibitors on S6 kinase activity.
  • Measured the timing of S6 kinase activation and phosphorylation following EGF treatment.
  • S6 kinase activity increased by up to 10 times with EGF stimulation (max observed at 15-30 min).
  • Only 20-30% of kinase activity remained after 2 hours, indicating transient activation.
  • Sodium orthovanadate also activates S6 kinase, but through an EGF receptor-independent pathway.

Cite This Study

Novak‐Hofer et al. (1985) studied this question.

synapsesocial.com/papers/6a0cd3369d761985b14a522chttps://doi.org/10.1016/s0021-9258(17)39249-9
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