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Polymerized actin hydrolyzes bound ATP in a reaction that depends on the concentration of polymerized ATP-actin, not on the rate of incorporation of ATP-actin into the polymer. The apparent first order rate constant is about 0.07 s-1 at 21 degrees C in 50 mM KCl with 1 mM MgCl2 or CaCl2.
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Pollard et al. (1984) studied this question.
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