Key result
Bovine profilin I mutant F59A, which has reduced actin affinity, was much less effective than wild-type profilin at rescuing growth and motility defects in profilin null Dictyostelium cells.
Population
Recombinant bovine profilin I proteins, fibroblasts, and Dictyostelium profilin null cells
Comparison
Point mutations in bovine profilin I vs Wild-type bovine profilin I
Design
Preclinical
Authors
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Profilin actin affinity is required for motility rescue in model cells; leaves open relevance to mammalian systems.
The study demonstrates that the actin-binding capacity of profilin is directly correlated with its biological functions in cell motility and proliferation.
Schlüter et al. (1998) studied this question. Bovine profilin I mutant F59A vs. Wild-type bovine profilin I was evaluated on Actin-binding affinity, growth of colony surface areas, and percentage of polynucleated cells. Bovine profilin I mutant F59A, which has reduced actin affinity, was much less effective than wild-type profilin at rescuing growth and motility defects in profilin null Dictyostelium cells.
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