Characterization of Actin and Poly-L-proline Binding Sites of Profilin with Monoclonal Antibodies and by Mutagenesis
Population
Acanthamoeba profilin (deletion and substitution mutations, and nine monoclonal antibodies)
Design
Preclinical
Authors
Discussion
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Member takes
Implication
C-terminal sensitivity in profilin warrants targeted mutagenesis in mammalian models; leaves open relevance to cardiac actin dynamics.
Structured PICO
P
Population
Acanthamoeba profilin (deletion and substitution mutations, and nine monoclonal antibodies)
I
Intervention
Mutagenesis and monoclonal antibody characterization
O
Outcome
Binding sites for actin and poly-L-proline on the profilin molecule
The study provides structural and mutagenic evidence characterizing the actin and poly-L-proline binding sites of Acanthamoeba profilin, highlighting the critical role of the C-terminus in structural stability.
Cite This Study
Thomas D. Pollard (1996) studied this question.