Key result
Treatment of monomer actin with 1 M MgCl2 increased the rotational correlation time from 16 ns to 26 ns, and addition of heavy meromyosin induced polymerization of the MgCl2-depolymerized actin.
Population
Actin monomer solutions (F-actin pellet treated with 1 M MgCl2)
Comparison
1 M MgCl2 treatment and addition of heavy… vs Normal actin solutions at low ionic strength
Design
Preclinical
Authors
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Should not change practice; leaves open extension to cardiac cytoskeletal research.
Absolute Event Rate: 26% vs 16%
High salt concentration increases the rotational correlation time of monomer actin, and heavy meromyosin can induce its polymerization.
Belágyi et al. (1983) studied Actin monomer solutions. 1 M MgCl2 vs. Normal actin solutions (low ionic strength) was evaluated on Rotational correlation time. Treatment of monomer actin with 1 M MgCl2 increased the rotational correlation time from 16 ns to 26 ns, and addition of heavy meromyosin induced polymerization of the MgCl2-depolymerized actin.
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