Key result
CRISPR screen identifies 60S ribosome biogenesis genes including SPATA5 as critical for viral replication.
Why the study?
Viruses co-opt host ribosomes to synthesize viral proteins, but the specific factors involved in translation of viral RNAs are not fully described.
Genome-scale CRISPR screening identified 60S ribosome biogenesis factors, such as SPATA5, as critical host factors for viral RNA translation and replication.
Viruses are well known for their ability to co-opt host ribosomes to synthesize viral proteins. The specific factors involved in translation of viral RNAs are not fully described. In this study, we implemented a unique genome-scale CRISPR screen to identify previously uncharacterized host factors that are important for the synthesis of virally encoded protein. We found that multiple genes involved in 60S ribosome biogenesis were required for viral RNA translation. Loss of these factors severely impaired viral replication. Mechanistic studies on the AAA ATPase SPATA5 indicate that this host factor is required for a late step in ribosome formation. These findings reveal insight into the identity and function of specific ribosome biogenesis proteins that are critical for viral infections.
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Ohlson et al. (2023) studied Viral infection. Genome-scale CRISPR screen was evaluated on Identification of host factors required for viral RNA translation and replication. A genome-scale CRISPR screen identified multiple genes involved in 60S ribosome biogenesis, including the AAA ATPase SPATA5, as critical host factors required for viral RNA translation and replication.
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