Key result
Alternatively treated pericardial valve material supported regular endothelial cell proliferation, whereas conventionally treated tissue caused cell death within 2 days.
Why the study?
Does alternative preservation with L-glutamic acid and collagen precoating improve endothelial cell viability and proliferation on bioprosthetic heart valve material?
Does alternative preservation with L-glutamic acid and collagen precoating improve endothelial cell viability and proliferation on bioprosthetic heart valve material?
Alternative preservation of bioprosthetic valves with L-glutamic acid and precoating with fibrillar collagen type I significantly improves endothelial cell attachment and proliferation in vitro.
Alternative preservation may enhance endothelial viability in vitro; leaves open effects on clinical valve durability.
In this in vitro study, the growth properties of cultured endothelial cells on conventionally treated pericardial valve material were measured. These data were compared to endothelial cell proliferation on an alternatively treated valve material. This alternative preservation procedure was developed in order to bind free, residual glutaraldehyde in the valve tissue by reaction with L-glutamic acid. In order to optimize endothelial cell attachment and proliferation, fibronectin and fibrillar collagen type I were tested as surface precoating substances. Cell viability of the seeded cells was evaluated by means of proliferation kinetics, antithrombotic activity, and morphological appearance. Endothelial cell death occurred within the first 2 days after seeding on conventionally treated valve tissue, independent of the type of precoating. On alternatively treated tissue, regular endothelial cell proliferation was observed. Precoating with fibrillar collagen markedly increased endothelial cell attachment and proliferation as compared to fibronectin. Maintenance of antithrombotic activity of the seeded cells was proven by regular release of prostacyclin.
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Grabenwöger et al. (1992) studied Bioprosthetic Heart Valve Material. Alternatively treated valve material (L-glutamic acid) and fibrillar collagen precoating vs. Conventionally treated pericardial valve material and fibronectin precoating was evaluated on Cell viability, proliferation kinetics, antithrombotic activity, and morphological appearance. Alternatively treated pericardial valve material supported regular endothelial cell proliferation, whereas conventionally treated tissue caused cell death within 2 days.
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