Key result
In vitro seeding of isolated porcine valve bioprosthesis cusps with cultured adult human vein endothelial cells resulted in a confluent lining at 7 days, whereas cells seeded on intact valves died.
Why the study?
Can commercially available porcine heart valve bioprostheses be successfully endothelialized in vitro using cultured adult human vein endothelial cells?
Can commercially available porcine heart valve bioprostheses be successfully endothelialized in vitro using cultured adult human vein endothelial cells?
While isolated porcine bioprosthetic valve cusps can be successfully endothelialized with human cells in vitro, intact valves show poor cell survival at 7 days, highlighting challenges in whole-valve tissue engineering.
No takes yet. Share an insight, caveat, or question.
Intact valves resist endothelialization unlike isolated cusps; leaves open feasibility of whole-valve tissue engineering.
Bengtsson et al. (1993) studied Heart valve bioprostheses. Preendothelialization with cultured adult human vein endothelial cells was evaluated on Endothelialization of isolated cusps and intact heart valve bioprostheses. In vitro seeding of isolated porcine valve bioprosthesis cusps with cultured adult human vein endothelial cells resulted in a confluent lining at 7 days, whereas cells seeded on intact valves died.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: