Effect of Guanxin V in animal model of acute myocardial infarction

Figures

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  Fig. 1. GXV effectively improved cardiac function in AMI hamsters. a Representative M-mode echocardiographic images of hamsters in Sham operation, AMI, GXV and Tra groups recorded in the 4th and 8th week, postoperatively (n = 6). b-i EF, FS, LVIDd, LVIDs, LVPWd, LVPWs, LVEDV and LVESV recorded in the 4th and 8th week, postoperatively (n = 6). *P < 0.05 v.s. Sham operation group; **P < 0.01 v.s. Sham operation group. #P < 0.05 v.s. AMI group; ##P < 0.01 v.s. AMI group

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  Fig. 2. Representative images of TTC staining and analysis of HWI. a The infarct size in AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 3). b HWI in AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 6). *P < 0.05 v.s. Sham operation group; **P < 0.01 v.s. Sham operation group. #P < 0.05 v.s. AMI group; ##P < 0.01 v.s. AMI group

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  Fig. 3. Representative images of H&E, Masson and immunohistochemical staining in myocardium (magnification 400×). a H&E staining of myocardium collected from hamsters in Sham operation, AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 3). b,e Masson staining of myocardium collected from hamsters in Sham operation, AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 6). Collagen fibers were stained green and myocardial fibers were red. (c-g) Immunohistochemical staining about positive expressions of Collagen I and Collagen III in myocardium collected from hamsters in Sham operation, AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 3). Collagen fibers were stained brown

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  Fig. 4. Relative levels of Renin, Ang I, Ang II, chymase and AT1R detected by ELISA, WB and IHC. a-d Relative levels of Renin, Ang I, Ang II and chymase in heart tissues collected from hamsters in Sham operation, AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 5). e-h Relative levels of Renin, Ang I, Ang II and chymase in serum collected from hamsters in Sham operation, AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 6). (i-k) Protein levels of chymase and AT1R in heart tissues collected from hamsters in Sham operation, AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 3). (l-o) Immunohistochemical staining on chymase and AT1R in heart tissues collected from hamsters in Sham operation, AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 3). Magnification: 400 × .*P < 0.05 v.s. Sham operation group; **P < 0.01 v.s. Sham operation group. #P < 0.05 v.s. AMI group; ##P < 0.01 v.s. AMI group

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  Fig. 5. Myocardium apoptosis evaluated by TUNEL assay (magnification 400×). (a, b) Representative images of TUNEL staining in Sham operation, AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 6). Apoptosis cells were stained brown

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  Fig. 6. The number of MCs detected by TB staining (magnification 400×). a, b Representative images of TB staining in Sham operation, AMI, GXV and Tra groups in the 4th and 8th week, postoperatively (n = 6). MCs were stained bluish violet