Key result
Isoproterenol alters specific primate serum proteins, indicating their potential as cardiac injury biomarkers.
Why the study?
Does isoproterenol treatment alter serum and heart tissue proteins to identify new cardiac biomarkers in nonhuman primates?
Does isoproterenol treatment alter serum and heart tissue proteins to identify new cardiac biomarkers in nonhuman primates?
Serum amyloid A, α-1-acid glycoprotein, and apolipoprotein A-1 are potential new biomarkers for drug-induced cardiac injury based on proteomic analysis in nonhuman primates.
Should not yet change clinical biomarker practice; leaves open human validation of these proteins for cardiac injury.
To identify new cardiac biomarkers, a quantitative proteomic analysis has been performed on serum and heart tissue proteins from three species of nonhuman primates following isoproterenol (ISO) treatment. Three serum proteins--serum amyloid A (SAA), α-1-acid glycoprotein (A1AG), and apolipoprotein A-1 (Apo A1)--were consistently identified as changed and remained altered 72 h post dose in all three species post ISO treatment, indicating the potential of including these proteins in preclinical or clinical evaluation of drug-induced cardiac injury. Furthermore, proteomic analysis of heart tissue proteins following ISO treatment demonstrated detrimental effects on calcium signaling and energy generation in cardiac myocytes. It is worth noting that cardiac troponins were not identified in serum but were identified as altered in heart tissue lysate along with other cardiac-specific proteins. This strategy for cardiac biomarker discovery by proteomic screening of heart tissue proteins, followed by verification in serum samples using immunoassays or targeted mass spectrometry, could be applied in future biomarker studies.
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Song et al. (2014) studied Drug-induced cardiac injury. Isoproterenol (ISO) was evaluated on Identification of altered serum and heart tissue proteins. Isoproterenol treatment in nonhuman primates altered serum amyloid A, α-1-acid glycoprotein, and apolipoprotein A-1 at 72 hours, indicating their potential as cardiac injury biomarkers.
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