Key result
Exposure of bovine aortic endothelial cells to 10% average cyclic strain resulted in a 1.5- to 2.2-fold increase in AC, cAMP, and PKA activity by 15 minutes compared to unstretched controls.
Population
Bovine aortic endothelial cells (EC) seeded to confluence on flexible membrane-bottom wells
Comparison
Mechanical strain at 150 mm Hg or 37.5 mm Hg… vs Unstretched controls
Design
Preclinical
Follow-up
0-60 min
Authors
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Hypothesis-generating for endothelial mechanotransduction; leaves open in vivo relevance and clinical translation.
Effect estimate: 1.5- to 2.2-fold increase
Cyclic strain exceeding a threshold (10%) activates the AC/cAMP/PKA signal transduction pathway in endothelial cells, potentially stimulating gene expression.
Cohen et al. (1997) studied Endothelial cells exposed to mechanical strain. 10% average cyclic strain (150 mm Hg vacuum) vs. 6% average strain (37.5 mm Hg vacuum) and unstretched controls was evaluated on AC, cAMP, and PKA activity (1.5- to 2.2-fold increase). Exposure of bovine aortic endothelial cells to 10% average cyclic strain resulted in a 1.5- to 2.2-fold increase in AC, cAMP, and PKA activity by 15 minutes compared to unstretched controls.
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