What question did this study set out to answer?

To establish and validate a label-free liquid chromatography-mass spectrometry method for quantifying proteomic and metabolomic profiles in biofluids without requiring isotopic labeling or internal standards.

August 8, 2003Analytical Chemistry

Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards

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Authors

WWWeixun WangUnited States Military Academy HZHaihong ZhouHainan University HLHua LinChinese Academy of Medical Sciences & Peking Union Medical College

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Implication

Methodological study demonstrates reproducible label-free quantification of proteins and metabolites in human serum, indicating viable biomarker discovery without isotopic standards.

Key Points

To establish and validate a label-free liquid chromatography-mass spectrometry method for quantifying proteomic and metabolomic profiles in biofluids without requiring isotopic labeling or internal standards.
Applied liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization to human serum samples.
Profiled approximately 3,400 molecular ions across 25 independently processed human serum samples, generating 85,000 individual molecular ion measurements without spiked standards or isotopic tags.
Observed linear standard curves for diverse compounds introduced into human serum based on signal linearity and processing reproducibility.
Achieved median analytical coefficients of variation of 25.7% for the proteome and 23.8% for the metabolome across 25 independently processed serum samples.

Cite This Study

Wang et al. (2003) studied this question.

synapsesocial.com/papers/6a0df66ccecdf5fb20baa91b https://doi.org/10.1021/ac026468x

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Implication

Methodological study demonstrates reproducible label-free quantification of proteins and metabolites in human serum, indicating viable biomarker discovery without isotopic standards.

Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards

Discussion

Member takes

Implication

Key Points

Cite This Study

Quantification of Proteins and Metabolites by Mass Spectrometry without Isotopic Labeling or Spiked Standards

Discussion

Member takes

Implication

Key Points

Cite This Study

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