Key result
Fluorescence imaging of Myosin VI revealed an average step size of ~60 nm for a labeled head, strongly supporting a hand-over-hand model of motility with the lever arm undergoing spatial fluctuations.
Population
porcine myosin VI cDNA expressed in Sf9 cells and chicken CaM expressed in E. coli
Design
Preclinical
Authors
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Hypothesis-generating for cardiac motor protein function; leaves open translation to human myosin VI in vivo.
Myosin VI moves via a hand-over-hand mechanism with an average step size of ~60 nm, and its lever arm undergoes spatial fluctuations during motility.
Yıldız et al. (2004) studied this question. FIONA (fluorescence imaging with one nanometer accuracy) was evaluated on Average step size of a labeled head. Fluorescence imaging of Myosin VI revealed an average step size of ~60 nm for a labeled head, strongly supporting a hand-over-hand model of motility with the lever arm undergoing spatial fluctuations.
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