What question did this study set out to answer?

This study evaluates the influence of trigger calcium and sarcoplasmic reticulum calcium content on fractional calcium release in cardiac myocytes.

May 1, 1995AJP Cell Physiology

Fractional SR Ca release is regulated by trigger Ca and SR Ca content in cardiac myocytes

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This study evaluates the influence of trigger calcium and sarcoplasmic reticulum calcium content on fractional calcium release in cardiac myocytes.
Isolated ferret ventricular myocytes were used for experimentation.
Use of indo 1 to measure calcium concentration and patch-clamp technique to measure calcium current.
Caffeine and thapsigargin were employed to manipulate SR calcium release and uptake.
At normal SR Ca load with 2 mM extracellular Ca, 35 +/- 3% fractional SR Ca was released; this changed to 10 +/- 2% and 59 +/- 6% with [Ca]o of 0.5 mM and 8 mM, respectively.
Increasing the SR Ca load at different stimulation frequencies resulted in only a 4% increase in content in the High-load condition.

Structured PICO

Population

Isolated ferret ventricular myocytes

Intervention

Alteration of trigger Ca (by changing extracellular Ca concentration to 0.5 and 8 mM) and alteration of SR Ca content (by action potential stimulation at different frequencies from 0.05 to 0.8 Hz)

Comparator

Normal SR Ca load and trigger Ca (action potential at 0.5 Hz with 2 mM extracellular Ca concentration)

Outcome

Fractional SR Ca releasesurrogate

Fractional SR Ca release in cardiac myocytes is highly dependent on the amount of trigger Ca and SR Ca content.

Discussion

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Implication

Hypothesis-generating for trigger Ca dependence of fractional SR release in myocytes; leaves open translation to human disease models.

Cite This Study

Bassani et al. (1995) studied this question.

synapsesocial.com/papers/6a0e9ece25c30b2cc7f99df8 https://doi.org/10.1152/ajpcell.1995.268.5.c1313

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Fractional SR Ca release is regulated by trigger Ca and SR Ca content in cardiac myocytes

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Fractional SR Ca release is regulated by trigger Ca and SR Ca content in cardiac myocytes

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider