Control of Maximum Sarcoplasmic Reticulum Ca Load in Intact Ferret Ventricular Myocytes
Population
Intact ferret ventricular myocytes (voltage-clamped)
Comparison
Acceleration of SR Ca pumping by isoproterenol… vs Control conditions
Design
Preclinical
Authors
Discussion
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Member takes
Implication
SR Ca stability in animal myocytes warrants no clinical changes; leaves open thermodynamic SERCA validation in intact or human models.
Structured PICO
P
Population
Intact ferret ventricular myocytes (voltage-clamped)
I
Intervention
Acceleration of SR Ca pumping by isoproterenol (1 micro M) or slowing by thapsigargin (0.2-0.4 micro M)
C
Comparator
Control conditions
O
Outcome
Maximal caffeine-released SR Ca contentsurrogate
The relative stability of the SR Ca load suggests that dissipative losses have only a minor influence in setting the SR Ca content, and the SR Ca pump in intact cells can generate a calcium gradient approaching the thermodynamic limit.
Cite This Study
Ginsburg et al. (1998) studied this question.