What question did this study set out to answer?

This research aims to understand how cardiac fibroblasts affect the health and functionality of cardiomyocytes through soluble mediators.

January 5, 2015ViewOpen Access

Functional crosstalk between cardiac fibroblasts and adult cardiomyocytes by soluble mediators

Q: What is the clinical evidence from this study?

Study design: Other. Population: Cardiac fibroblast and cardiomyocyte crosstalk. Intervention: Fibroblast conditioned medium and co-culture vs. Different fibroblast phenotypes. Primary outcome: Cardiomyocyte viability, volume, and Ca(2+) transient amplitude.

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Key result

Fibroblast paracrine signaling differentially regulates cardiomyocyte viability and calcium handling via TGF-β.

Population

Adult cardiomyocytes, freshly isolated fibroblasts, and cultured fibroblasts from normal rat hearts, and…

Comparison

Co-culture methods and conditioned medium, with… vs Different fibroblast phenotypes and unblocked…

Design

Preclinical

Authors

JCJames CartledgeBarts Health NHS Trust CKChristopher KaneUC San Diego Health System PDPriyanthi DiasQueen Mary University of London

Discussion

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Implication

May inform TGF-β targeting in cardiac remodeling models; leaves open translation to human therapies.

Key Points

This research aims to understand how cardiac fibroblasts affect the health and functionality of cardiomyocytes through soluble mediators.
Used co-culture methods and conditioned medium to study fibroblast-cardiomyocyte interactions.
Compared effects of freshly isolated and cultured fibroblasts on adult cardiomyocyte viability and calcium handling.
Examined the role of TGF-β in fibroblast-cardiomyocyte signaling.
Fibroblasts decrease cardiomyocyte viability and increase volume; α-SMA-negative fibroblasts enhance Ca(2+) transient amplitude.
Cultured fibroblasts and myofibroblasts from pressure-overloaded hearts reduce Ca(2+) transient amplitude.
TGF-β promotes cardiomyocyte hypertrophy and mediates signaling between cell types.

Structured PICO

Population

Adult cardiomyocytes, freshly isolated fibroblasts, and cultured fibroblasts from normal rat hearts, and myofibroblasts from pressure-overloaded rat hearts

Intervention

Co-culture methods and conditioned medium, with or without TGF-β type 1 receptor blockade using SB431542

Comparator

Different fibroblast phenotypes (freshly isolated vs. cultured vs. pressure-overloaded) and unblocked conditions

Outcome

Cardiomyocyte viability, volume, Ca(2+) transient amplitude, and fibroblast proliferationsurrogate

Fibroblasts and cardiomyocytes engage in bi-directional crosstalk mediated by soluble factors like TGF-β, which differentially affects cardiomyocyte function and hypertrophy depending on the fibroblast phenotype.

Cite This Study

Cartledge et al. (2015) studied Cardiac fibroblast and cardiomyocyte crosstalk. Fibroblast conditioned medium and co-culture vs. Different fibroblast phenotypes was evaluated on Cardiomyocyte viability, volume, and Ca(2+) transient amplitude. Paracrine signaling from different fibroblast phenotypes differentially regulates adult cardiomyocyte viability, volume, and calcium handling, with TGF-β playing a crucial role in this crosstalk.

synapsesocial.com/papers/6a0ecf60a14f152feaf9dee5 https://doi.org/10.1093/cvr/cvu264

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Functional crosstalk between cardiac fibroblasts and adult cardiomyocytes by soluble mediators

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Functional crosstalk between cardiac fibroblasts and adult cardiomyocytes by soluble mediators

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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