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August 3, 1992FEBS LettersOpen Access

Molecular cloning and characterization of human endothelial nitric oxide synthase

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Key result

Molecular cloning of human endothelial nitric oxide synthase revealed a 1,203 amino acid protein sharing 94% identity with bovine endothelial NOS but only 60% with the rat brain isoform.

Population

Human umbilical vein endothelial cells (HUVEC) and vascular endothelium

Comparison

Molecular cloning and sequence analysis of human… vs Bovine endothelial NO synthase and rat brain NO…

Design

Preclinical

Authors

PMPhilip A. MarsdenOntario Institute for Cancer ResearchKSKeith SchappertSt. Michael's HospitalHCHai Sheine ChenUniversity of Toronto

Discussion

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Implication

Human eNOS cloning distinguishes it from neuronal NOS; leaves open isoform-specific vascular targeting.

Structured PICO

P
Population
Human umbilical vein endothelial cells (HUVEC) and vascular endothelium
I
Intervention
Molecular cloning and sequence analysis of human endothelial NO synthase
C
Comparator
Bovine endothelial NO synthase and rat brain NO synthase isoform
O
Outcome
Protein sequence identity and mRNA transcript localization

The cloning of human endothelial nitric oxide synthase demonstrated its distinct molecular identity and specific expression in vascular endothelium compared to neuronal isoforms.

Cite This Study

Marsden et al. (1992) studied this question. Molecular cloning of human endothelial nitric oxide synthase revealed a 1,203 amino acid protein sharing 94% identity with bovine endothelial NOS but only 60% with the rat brain isoform.

synapsesocial.com/papers/6a0ef4807046b28dbef9af46https://doi.org/10.1016/0014-5793(92)80697-f
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Also Consider

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  1. 1Calmodulin-dependent endothelium-derived relaxing factor/nitric oxide synthase activity is present in the particulate and cytosolic fractions of bovine aortic endothelial cells.1991 · 594 citations
  2. 2Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme.1990 · 3,307 citations
  3. 3Nitric oxide synthesis in endothelial cells: evidence for a pathway inducible by TNF-alpha1991 · 200 citations
  4. 4Tumor necrosis factor alpha activates soluble guanylate cyclase in bovine glomerular mesangial cells via an L-arginine-dependent mechanism.1990 · 130 citations
  5. 5Transcriptional regulation of the endothelin-1 gene by TNF-alpha1992 · 288 citations