Cardiac activation–repolarization patterns and ion channel expression mapping in intact isolated normal human hearts
Key result
Normal human heart repolarization gradients span all axes without late midmural repolarization or ion channel mRNA correlation.
- n=3
Population
Isolated hearts from 3 donors without reported cardiac disease, Langendorff perfused with the patient's own…
Design
Preclinical
Authors
Discussion
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Implication
Does not support late midmural repolarization in normal hearts; leaves open ion channel mRNA roles in arrhythmogenesis.
Structured PICO
P
Population
Isolated hearts from 3 donors without reported cardiac disease, Langendorff perfused with the patient's own blood
I
Intervention
Multisite analysis of activation-repolarization patterns (up to 92 unipolar electrograms from 24 transmural needles) and mRNA expression patterns of ion channel subunits (up to 63 sites)
O
Outcome
Activation-repolarization patterns, activation-recovery intervals (ARI), and mRNA levels of ion channel subunitssurrogate
In normal human hearts, repolarization gradients encompass all axes without late midmural repolarization, and local mRNA expression of ion channels does not correlate with local activation-recovery intervals.
Cite This Study
Opthof et al. (2016) studied Normal human hearts (n=3). Multisite analysis of activation-repolarization patterns and mRNA expression was evaluated on Repolarization gradients, activation-repolarization relationship, and mRNA expression patterns. In normal isolated human hearts, repolarization gradients encompass all axes without late midmural repolarization, and gradients of mRNAs of single ion channel subunits do not correlate with ARIs.