The whole transcriptome and proteome changes in the early stage of myocardial infarction

Figures

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  Fig. 1. Functional evaluation of myocardial infarction mouse model. a Schematic diagram of this study. b Schematic diagram of the mouse MI model and infarct tissue. c The Masson staining of myocardial tissues. The arrow shows the fibrosis. Bar = 100 μm

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  Fig. 2. Transcriptome analysis of different time points of MI. a Principal component analysis of control and MI groups. b Volcano plots of the differentially expressed genes between the control group and MI groups. c Venn diagram of differentially expressed genes between the control group and MI group. d Heatmap of the top 200 differentially expressed genes between the control group and MI groups

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  Fig. 3. Immunity- and proliferation-related genes are significantly increased after MI. a GO biological process analysis of the differentially expressed genes between the control group and the 24 h group. b GSEA analysis of transcriptomic gene expression between the control group and the 24 h group. c GO biological process analysis of the differentially expressed genes between the control group and the 72 h group. d GSEA analysis of transcriptomic gene expression between the control group and the 72 h group. NES normalised enrichment score, P nominal P value, Padj adjusted P value

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  Fig. 4. Immune cell infiltration during myocardial infarction. a Heatmap of molecular markers specific to different immune cell populations. b mRNA z-scores of monocytes and macrophages. c mRNA z-scores of naive and active B cells. d mRNA z-scores of naive and active T cells

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  Fig. 5. Proteome analysis of different time points of MI. a Volcano plots of the differentially expressed proteins between the control group and MI groups. b Venn diagram of the differentially expressed proteins between the control group and MI groups. c Heatmap of the co-differentially expressed proteins between the control group and both MI groups (24 and 72 h). d Functional annotation of the co-differentially expressed proteins between the control group and both MI groups (24 and 72 h) via the DAVID database

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  Fig. 6. BFLF2 is a nucleocytoplasmic shuttling protein. a Venn diagram analysis of the common differential genes in the 24 h group. b Venn diagram analysis of the common differential genes in the 72 h group. c Differential expression of Serpina3n, Anxa1, and Nppa in the transcriptome and proteome at different time points after MI. d qPCR analysis of Serpina3n, Anxa1, and Nppa at different time points after MI (values are mean ± SD, *P < 0.05, two-tailed Student’s t-test)