What question did this study set out to answer?

To determine whether inflammatory cytokines stimulate human endothelial cells to synthesize and secrete the monocyte chemoattractant MCP-1/JE.

June 1, 1990Open Access

Cytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE.

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Authors

BRBarrett J. RollinsBrigham and Women's Hospital TYTeizo YoshimuraFrederick National Laboratory for Cancer Research ELEdward J. LeonardNational Institutes of Health

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Implication

In vitro study reveals that inflammatory cytokines trigger endothelial cells to secrete MCP-1, indicating a key role for blood vessel linings in monocyte recruitment during inflammation.

Key Points

To determine whether inflammatory cytokines stimulate human endothelial cells to synthesize and secrete the monocyte chemoattractant MCP-1/JE.
Exposed cultured human endothelial cells to the inflammatory cytokines interleukin-1 beta (IL-1 beta), tumor necrosis factor (TNF), and interferon-gamma (IFN-gamma).
Monitored MCP-1 mRNA expression kinetics over 3 to 24 hours and measured protein accumulation in culture medium across a 48-hour period.
Assessed monocyte chemoattractant bioactivity of conditioned medium before and after immunoadsorption with anti-MCP-1 antibodies.
Treatment with IL-1 beta and TNF induced robust MCP-1 mRNA transcription within 3 hours, whereas IFN-gamma stimulated weaker expression detectable after 24 hours.
MCP-1/JE protein continuously accumulated in the culture supernatant during 48 hours of sustained IL-1 beta stimulation.
Conditioned medium exhibited potent monocyte chemoattractant activity that was specifically neutralized and removed via immunoadsorption with anti-MCP-1 antibodies.

Cite This Study

Rollins et al. (1990) studied this question.

synapsesocial.com/papers/6a0fe2cfd8c5cf602efd4b5f https://doi.org/10.1136/bmj.2.4930.12

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Implication

In vitro study reveals that inflammatory cytokines trigger endothelial cells to secrete MCP-1, indicating a key role for blood vessel linings in monocyte recruitment during inflammation.

Cytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE.

Discussion

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Implication

Key Points

Cite This Study

Cytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE.

Discussion

Member takes

Implication

Key Points

Cite This Study

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