What is the clinical evidence from this study?

Population: Diabetes mellitus. Intervention: Acetylcholine, A23187, sodium nitroprusside, adenosine vs. Control rats. Primary outcome: Endothelium-dependent and endothelium-independent relaxation in thoracic aorta.

What question did this study set out to answer?

To determine whether spontaneous diabetes alters endothelium-dependent versus endothelium-independent vasorelaxation in rat aortic tissue.

June 1, 1988British Journal of PharmacologyOpen Access

Impairment of endothelium‐dependent relaxation in aortae from spontaneously diabetic rats

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Key result

Endothelium-dependent relaxation produced by acetylcholine or A23187 was significantly attenuated in diabetic rat aortae compared to controls, while endothelium-independent relaxation was preserved.

Why the study?

Does diabetes impair endothelium-dependent relaxation in rat aortae?

Population

Thoracic aortae from spontaneously diabetic rats and control rats

Comparison

Endothelium-dependent relaxing substances and… vs Aortae from control (non-diabetic) rats

Design

Preclinical

Authors

WDWilliam DuranteUniversity of Missouri ASAmar K. SenGreenfield Research (Canada)FSF. A. SunaharaUniversity of Toronto

Discussion

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Implication

Endothelial impairment may underlie diabetic vascular changes in this model; leaves open translation to human disease and therapies.

Key Points

To determine whether spontaneous diabetes alters endothelium-dependent versus endothelium-independent vasorelaxation in rat aortic tissue.
Isolated thoracic aortic rings from control and spontaneously diabetic rats and precontracted them with phenylephrine.
Assessed endothelium-dependent relaxation using acetylcholine and the calcium ionophore A23187.
Evaluated endothelium-independent relaxation using sodium nitroprusside and adenosine.
Endothelium-dependent relaxation triggered by acetylcholine or A23187 was significantly attenuated in diabetic aortic rings compared with controls.
Endothelium-independent relaxation induced by sodium nitroprusside or adenosine showed no impairment in diabetic vessels relative to controls.

Structured PICO

Does diabetes impair endothelium-dependent relaxation in rat aortae?

Population

Thoracic aortae from spontaneously diabetic rats and control rats

Intervention

Endothelium-dependent relaxing substances (acetylcholine, calcium ionophore A23187) and endothelium-independent relaxing substances (sodium nitroprusside, adenosine)

Comparator

Aortae from control (non-diabetic) rats

Outcome

Vascular relaxation (endothelium-dependent and endothelium-independent)surrogate

Diabetes leads to a specific impairment of endothelium-dependent relaxation in rat aortae, highlighting a mechanism for vascular reactivity alterations in diabetes.

Cite This Study

Durante et al. (1988) studied Diabetes mellitus. Acetylcholine, A23187, sodium nitroprusside, adenosine vs. Control rats was evaluated on Endothelium-dependent and endothelium-independent relaxation in thoracic aorta. Endothelium-dependent relaxation produced by acetylcholine or A23187 was significantly attenuated in diabetic rat aortae compared to controls, while endothelium-independent relaxation was preserved.

synapsesocial.com/papers/6a0fef6f92676d5461fd499e https://doi.org/10.1111/j.1476-5381.1988.tb11548.x

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Impairment of endothelium‐dependent relaxation in aortae from spontaneously diabetic rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Impairment of endothelium‐dependent relaxation in aortae from spontaneously diabetic rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider