May 1, 1993Journal of Clinical MicrobiologyOpen Access

Detection of hog cholera virus and differentiation from other pestiviruses by polymerase chain reaction

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Population

Pestivirus isolates and tissue extracts

Comparison

Reverse transcription coupled with the… vs Virus propagation in tissue culture and…

Design

Preclinical

Authors

BWBarbara WirzInstitute of Virology of the Slovak Academy of Sciences JTJon Duri TratschinBoard of the Swiss Federal Institutes of Technology HMHeinz K. MüllerIndustrial Union of Metalworkers

Discussion

Member takes

Implication

May aid veterinary pestivirus detection in tissues; leaves open clinical translation without further validation.

Structured PICO

Population

Pestivirus isolates (hog cholera virus, bovine viral diarrhea virus, border disease virus) and tissue extracts (lymph node, spleen, tonsil, lung)

Intervention

Reverse transcription coupled with the polymerase chain reaction (RT-PCR) and multiplex PCR using specific primer pairs (PEST 1-PEST 2, HCV 1-HCV 2)

Comparator

Virus propagation in tissue culture and subsequent detection by immunofluorescence staining

Outcome

Detection and differentiation of pestivirusessurrogate

RT-PCR and multiplex PCR provide a rapid and sensitive method for detecting and differentiating hog cholera virus from other pestiviruses directly from tissue extracts.

Cite This Study

Wirz et al. (1993) studied this question.

synapsesocial.com/papers/6a0ff0d357bfcc72645fc61f https://doi.org/10.1128/jcm.31.5.1148-1154.1993

View Full Paper

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Detection of hog cholera virus and differentiation from other pestiviruses by polymerase chain reaction

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Detection of hog cholera virus and differentiation from other pestiviruses by polymerase chain reaction

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider