March 1, 1993Journal of Clinical MicrobiologyOpen Access

Presumptive diagnostic differentiation of hog cholera virus from bovine viral diarrhea and border disease viruses by using a cDNA nested-amplification approach

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Population

61 pestivirus isolates

Design

Preclinical

Authors

JKJonathan B. KatzScience Oxford JRJulia F. RidpathNational Animal Disease Center SBSteve BolinMichigan State University

Discussion

Member takes

Implication

May support specific PCR for pestivirus differentiation in veterinary settings; leaves open prospective clinical validation before diagnostic adoption.

Structured PICO

Population

61 pestivirus isolates (36 hog cholera virus [HCV], 25 bovine viral diarrhea virus [BVDV] and border disease virus [BDV])

Intervention

Nested polymerase chain reactions (PCRs) targeting two pestiviral genomic segments (5' genomic terminus and major envelope glycoprotein)

Outcome

Identification and differentiation of HCV from BVDV and BDV

A nested PCR approach can successfully differentiate hog cholera virus from related pestiviruses, aiding in rapid diagnostic surveillance.

Cite This Study

Katz et al. (1993) studied this question.

synapsesocial.com/papers/6a715b3d5d37378ac1de83bd https://doi.org/10.1128/jcm.31.3.565-568.1993

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Implication

May support specific PCR for pestivirus differentiation in veterinary settings; leaves open prospective clinical validation before diagnostic adoption.

Presumptive diagnostic differentiation of hog cholera virus from bovine viral diarrhea and border disease viruses by using a cDNA nested-amplification approach

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Presumptive diagnostic differentiation of hog cholera virus from bovine viral diarrhea and border disease viruses by using a cDNA nested-amplification approach

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider