Dose-response of a fibrolytic enzyme in dairy cows fed a sorghum silage-based diet: effects on productive performance, ruminal fermentation, and in vitro gas kinetics

) concentration in rumen fluid decreased following a quadratic pattern with increasing dose, and VFA concentration showed a cubic response. Gas production (mL gas/g of DM and OM incubated) increased linearly with dose, as well as the maximum gas production parameter. Gas production rate and lag time were not affected by treatment. Organic matter, DM and NDF digestibility at 48 and 96 h increased following a linear and cubic pattern with EFE doses. Further research is warranted to elucidate the mechanisms of action of EFE in dairy cows fed sorghum silage-based diets.