October 1, 1998Journal of Biological ChemistryOpen Access

Superoxide Generation from Endothelial Nitric-oxide Synthase

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Population

Purified endothelial nitric-oxide synthase (eNOS)

Design

Preclinical

Authors

YXYong XiaThe Affiliated Yongchuan Hospital of Chongqing Medical University ATAh-Lim TsaiThe University of Texas Health Science Center at Houston VBVladimír BerkaThe University of Texas Health Science Center at Houston

Discussion

Member takes

Implication

No immediate clinical implications from purified enzyme data; leaves open BH4 regulation of eNOS superoxide in intact vascular models.

Structured PICO

Population

Purified endothelial nitric-oxide synthase (eNOS)

Intervention

Various biochemical conditions including absence/presence of tetrahydrobiopterin (BH4), Ca2+/calmodulin, L-NAME, D-NAME, cyanide, imidazole, and L-arginine

Outcome

Superoxide (O-2) generation measured by electron paramagnetic resonance spin-trappingsurrogate

Purified eNOS can generate superoxide in a Ca2+/calmodulin-dependent manner that is primarily regulated by tetrahydrobiopterin (BH4).

Cite This Study

Xia et al. (1998) studied this question.

synapsesocial.com/papers/6a10177ffb2817e31dfceddc https://doi.org/10.1074/jbc.273.40.25804

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Superoxide Generation from Endothelial Nitric-oxide Synthase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Superoxide Generation from Endothelial Nitric-oxide Synthase

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider